Franz Hagn

Scientific Report on TUM-IAS Fellowship 

The TUM-IAS Focus Group Structural Membrane Biochemistry works on the development of tools for the high-resolution structure determination of membrane proteins in a native lipid bilayer environment by nuclear magnetic resonance (NMR) spectroscopy and other structural methods. Since membrane proteins are very difficult to study with structural methods, advanced strategies are required. full report

Short CV

  • Since 10/2014
    Rudolf Mößbauer Tenure Track Assistant Professor, Structural Membrane Biochemistry, Technische Universität München
  • 07/2010 -09/2014
    Postdoctoral Associate, Harvard Medical School, Boston, USA.
    Advisor: Prof. Dr. Gerhard Wagner, “Structural and functional studies of membrane proteins in phospholipid nanodiscs”
  • 11/2009-06/2010
    Postdoctoral Associate, Institute of Organic Chemistry and Biochemistry, Technische Universität München, Germany.
    Advisor: Prof. Dr. Horst Kessler, “NMR Spectroscopy of Molecular Chaperones, the p53 Tumorsuppressor Network and Spider Silk Proteins”
  • 01/2004-10/2009
    Doctoral research, Institute of Organic Chemistry and Biochemistry, Technische Universität München, Germany.
    Advisor: Prof. Dr. Horst Kessler „NMR Spectroscopy of Molecular Chaperones, the p53 Tumorsuppressor Network and Spider Silk Proteins“
    Oct. 2009: Dr. rer. nat. (grade: summa cum laude)
  • 08/2003-11/2003
    Intern, Aventis Pharma Germany GmbH, Frankfurt am Main at the division Drug Innovation and Approval, Chemistry.
    “Binding studies of compounds to receptors and enzymes by NMR spectroscopy”
  • 10/2002-7/2003
    Diploma, Institute of Biochemistry, Universität Bayreuth, Germany
    Advisor: Prof. Dr. Jochen Balbach, (grade: summa cum laude)
  • 1998-2003
    Study of Biochemistry, Universität Bayreuth, Germany

Selected Awards

  • 2015, Helmholtz Young Investigators‘ Group
  • 2013, TUM-IAS Hans Fischer Fellowship
  • 2012, Arnold-Sommerfeld Award of the Bavarian Academy of Sciences
  • 2011, Hans-Fischer Award 2011, Chemistry Department, Technische Universität München
  • 2011, Friedrich-Weygand Award 2011 of the Max-Bergmann Kreis
  • 2011, Human Frontier Science Program (HFSP) long-term fellowship
  • 2010, EMBO long-term fellowship
  • 2010, Participation and invited talk at the Nobel-Laureates’ Meeting, Lindau, Germany

Research Interests

Prof. Hagn's research is focused on nuclear magnetic resonance (NMR) spectroscopy of membrane proteins. This protein class is essential for signal transduction and the transfer of proteins and small molecules across the biological membrane barrier. The key to understanding these processes is to study these systems in a native environment provided by a phospholipid bilayer. Biologically relevant systems of interest are mitochondrial membrane proteins, G-protein coupled receptors (GPCRs) and their associated G-proteins, which are involved in metabolic diseases, neurological disorders and cancer.


Selected Publications

  • Nasr, Mahmoud L; Baptista, Diego; Strauss, Mike; Sun, Zhen-Yu J; Grigoriu, Simina; Huser, Sonja; Plückthun, Andreas; Hagn, Franz; Walz, Thomas; Hogle, James M; Wagner, Gerhard: Covalently circularized nanodiscs for studying membrane proteins and viral entry. Nature Methods 14 (1), 2016, 49-52
  • Etzkorn, Manuel; Raschle, Thomas; Hagn, Franz; Gelev, Vladimir; Rice, Amanda J.; Walz, Thomas; Wagner, Gerhard: Cell-free Expressed Bacteriorhodopsin in Different Soluble Membrane Mimetics: Biophysical Properties and NMR Accessibility. Structure 21, 2013, 394-401
  • Hagn, Franz; Etzkorn, Manuel; Raschle, Thomas; Wagner, Gerhard: Optimized Phospholipid Bilayer Nanodiscs Facilitate High-Resolution Structure Determination of Membrane Proteins. J. Am. Chem. Soc. 135, 2013, 1919-1925
  • Demmer, Oliver; Frank, Andreas O.; Hagn, Franz; Schottelius, Margret; Marinelli, Luciana; Cosconati, Sandro; Brack-Werner, Ruth; Kremb, Stephan; Wester, Hans-Jürgen; Kessler, Horst: A Conformationally Frozen Peptoid Boosts CXCR4 Affinity and Anti-HIV Activity. Angewandte Chemie International Edition 51 (32), 2012, 8110-8113
  • Hagn, Franz: A structural view on spider silk proteins and their role in fiber assembly. Journal of Peptide Science 18, 2012, 357-365
  • Hagn, Franz; Lagleder, Stephan; Retzlaff, Marco; Rohrberg, Julia; Demmer, Oliver; Richter, Klaus; Buchner, Johannes; Kessler, Horst: Structural analysis of the interaction between Hsp90 and the tumor suppressor protein p53. Nat Struct Mol Biol 18 (10), 2011, 1086-1093
  • Hagn, Franz; Thamm, Christopher; Scheibel, Thomas; Kessler, Horst: pH-Dependent Dimerization and Salt-Dependent Stabilization of the N-terminal Domain of Spider Dragline Silk-Implications for Fiber Formation. Angewandte Chemie International Edition 50, 2010, 310-313
  • Hagn, Franz; Eisoldt, Lukas; Hardy, John G.; Vendrely, Charlotte; Coles, Murray; Scheibel, Thomas; Kessler, Horst: A conserved spider silk domain acts as a molecular switch that controls fibre assembly. Nature 465 (7295), 2010, 239-242
  • Retzlaff, Marco; Hagn, Franz; Mitschke, Lars; Hessling, Martin; Gugel, Frederik; Kessler, Horst; Richter, Klaus; Buchner, Johannes: Asymmetric Activation of the Hsp90 Dimer by Its Cochaperone Aha1. Molecular Cell 37, 2010, 344-354
  • Hagn, F.; Klein, C.; Demmer, O.; Marchenko, N.; Vaseva, A.; Moll, U. M.; Kessler, H.: BclxL Changes Conformation upon Binding to Wild-type but Not Mutant p53 DNA Binding Domain. Journal of Biological Chemistry 285, 2009, 3439-3450

Publications as TUM-IAS-Fellow

2019

  • Hassan, Lara; Lin, Liangcai; Sorek, Hagit; Sperl, Laura E.; Goudoulas, Thomas; Hagn, Franz; Germann, Natalie; Tian, Chaoguang; Benz, J. Philipp: Crosstalk of Cellulose and Mannan Perception Pathways Leads to Inhibition of Cellulase Production in Several Filamentous Fungi. mBio 10 (4), 2019 mehr… BibTeX Volltext ( DOI )
  • Klöpfer, Kai; Hagn, Franz: Beyond detergent micelles: The advantages and applications of non-micellar and lipid-based membrane mimetics for solution-state NMR. Progress in Nuclear Magnetic Resonance Spectroscopy 114-115, 2019, 271-283 mehr… BibTeX Volltext ( DOI )
  • Reif, Maria M.; Fischer, Moritz; Fredriksson, Kai; Hagn, Franz; Zacharias, Martin: The N-Terminal Segment of the Voltage-Dependent Anion Channel: A Possible Membrane-Bound Intermediate in Pore Unbinding. Journal of Molecular Biology 431 (2), 2019, 223-243 mehr… BibTeX Volltext ( DOI )
  • Zeng, Baisen; Mou, Tung-Chung; Doukov, Tzanko I.; Steiner, Andrea; Yu, Wenxi; Papasergi-Scott, Makaia; Tall, Gregory G.; Hagn, Franz; Sprang, Stephen R.: Structure, Function, and Dynamics of the Gα Binding Domain of Ric-8A. Structure 27 (7), 2019, 1137-1147.e5 mehr… BibTeX Volltext ( DOI )

2018

  • Goricanec, David; Hagn, Franz: NMR backbone and methyl resonance assignments of an inhibitory G-alpha subunit in complex with GDP. Biomolecular NMR Assignments 13 (1), 2018, 131-137 mehr… BibTeX Volltext ( DOI )
  • Goricanec, David; Hagn, Franz: NMR backbone and methyl resonance assignments of an inhibitory G-alpha subunit in complex with GDP. Biomolecular NMR Assignments 13 (1), 2018, 131-137 mehr… BibTeX Volltext ( DOI )
  • Miehling, Jonas; Goricanec, David; Hagn, Franz: A Split-Intein-Based Method for the Efficient Production of Circularized Nanodiscs for Structural Studies of Membrane Proteins. ChemBioChem 19 (18), 2018, 1927-1933 mehr… BibTeX Volltext ( DOI )
  • Raltchev, Kolio; Pipercevic, Joka; Hagn, Franz: Production and Structural Analysis of Membrane-Anchored Proteins in Phospholipid Nanodiscs. Chemistry - A European Journal 24 (21), 2018, 5493-5499 mehr… BibTeX Volltext ( DOI )

2017

  • Hagn, Franz; Nasr, Mahmoud L; Wagner, Gerhard: Assembly of phospholipid nanodiscs of controlled size for structural studies of membrane proteins by NMR. Nature Protocols 13 (1), 2017, 79-98 mehr… BibTeX Volltext ( DOI )
  • Rodriguez Camargo, Diana C; Korshavn, Kyle J; Jussupow, Alexander; Raltchev, Kolio; Goricanec, David; Fleisch, Markus; Sarkar, Riddhiman; Xue, Kai; Aichler, Michaela; Mettenleiter, Gabriele; Walch, Axel Karl; Camilloni, Carlo; Hagn, Franz; Reif, Bernd; Ramamoorthy, Ayyalusamy: Stabilization and structural analysis of a membrane-associated hIAPP aggregation intermediate. eLife 6, 2017 mehr… BibTeX Volltext ( DOI )

2016

  • Enthart, Andreas; Klein, Christian; Dehner, Alexander; Coles, Murray; Gemmecker, Gerd; Kessler, Horst; Hagn, Franz: Solution structure and binding specificity of the p63 DNA binding domain. Scientific Reports 6 (1), 2016 mehr… BibTeX Volltext ( DOI )
  • Goricanec, David; Stehle, Ralf; Egloff, Pascal; Grigoriu, Simina; Plückthun, Andreas; Wagner, Gerhard; Hagn, Franz: Conformational dynamics of a G-protein α subunit is tightly regulated by nucleotide binding. Proceedings of the National Academy of Sciences 113 (26), 2016, E3629-E3638 mehr… BibTeX Volltext ( DOI )
  • Nasr, Mahmoud L; Baptista, Diego; Strauss, Mike; Sun, Zhen-Yu J; Grigoriu, Simina; Huser, Sonja; Plückthun, Andreas; Hagn, Franz; Walz, Thomas; Hogle, James M; Wagner, Gerhard: Covalently circularized nanodiscs for studying membrane proteins and viral entry. Nature Methods 14 (1), 2016, 49-52 mehr… BibTeX Volltext ( DOI )

2015

  • Milbradt, Alexander G.; Arthanari, Haribabu; Takeuchi, Koh; Boeszoermenyi, Andras; Hagn, Franz; Wagner, Gerhard: Increased resolution of aromatic cross peaks using alternate 13C labeling and TROSY. Journal of Biomolecular NMR 62 (3), 2015, 291-301 mehr… BibTeX Volltext ( DOI )

2014

  • Hagn, Franz; Wagner, Gerhard: Structure refinement and membrane positioning of selectively labeled OmpX in phospholipid nanodiscs. Journal of Biomolecular NMR 61 (3-4), 2014, 249-260 mehr… BibTeX Volltext ( DOI )
  • Linser, Rasmus; Gelev, Vladimir; Hagn, Franz; Arthanari, Haribabu; Hyberts, Sven G.; Wagner, Gerhard: Selective Methyl Labeling of Eukaryotic Membrane Proteins Using Cell-Free Expression. Journal of the American Chemical Society 136 (32), 2014, 11308-11310 mehr… BibTeX Volltext ( DOI )

2013

  • Etzkorn, Manuel; Raschle, Thomas; Hagn, Franz; Gelev, Vladimir; Rice, Amanda J.; Walz, Thomas; Wagner, Gerhard: Cell-free Expressed Bacteriorhodopsin in Different Soluble Membrane Mimetics: Biophysical Properties and NMR Accessibility. Structure 21, 2013, 394-401 mehr… BibTeX Volltext ( DOI )
  • Hagn, Franz; Etzkorn, Manuel; Raschle, Thomas; Wagner, Gerhard: Optimized Phospholipid Bilayer Nanodiscs Facilitate High-Resolution Structure Determination of Membrane Proteins. J. Am. Chem. Soc. 135, 2013, 1919-1925 mehr… BibTeX Volltext ( DOI )