The TUM-IAS Focus Group Structural Membrane Biochemistry works on the development of tools for the high-resolution structure determination of membrane proteins in a native lipid bilayer environment by nuclear magnetic resonance (NMR) spectroscopy and other structural methods. Since membrane proteins are very difficult to study with structural methods, advanced strategies are required. full report
Short CV
Since 10/2014 Rudolf Mößbauer Tenure Track Assistant Professor, Structural Membrane Biochemistry, Technische Universität München
07/2010 -09/2014 Postdoctoral Associate, Harvard Medical School, Boston, USA. Advisor: Prof. Dr. Gerhard Wagner, “Structural and functional studies of membrane proteins in phospholipid nanodiscs”
11/2009-06/2010 Postdoctoral Associate, Institute of Organic Chemistry and Biochemistry, Technische Universität München, Germany. Advisor: Prof. Dr. Horst Kessler, “NMR Spectroscopy of Molecular Chaperones, the p53 Tumorsuppressor Network and Spider Silk Proteins”
01/2004-10/2009 Doctoral research, Institute of Organic Chemistry and Biochemistry, Technische Universität München, Germany. Advisor: Prof. Dr. Horst Kessler „NMR Spectroscopy of Molecular Chaperones, the p53 Tumorsuppressor Network and Spider Silk Proteins“ Oct. 2009: Dr. rer. nat. (grade: summa cum laude)
08/2003-11/2003 Intern, Aventis Pharma Germany GmbH, Frankfurt am Main at the division Drug Innovation and Approval, Chemistry. “Binding studies of compounds to receptors and enzymes by NMR spectroscopy”
10/2002-7/2003 Diploma, Institute of Biochemistry, Universität Bayreuth, Germany Advisor: Prof. Dr. Jochen Balbach, (grade: summa cum laude)
1998-2003 Study of Biochemistry, Universität Bayreuth, Germany
Selected Awards
2015, Helmholtz Young Investigators‘ Group
2013, TUM-IAS Hans Fischer Fellowship
2012, Arnold-Sommerfeld Award of the Bavarian Academy of Sciences
2011, Hans-Fischer Award 2011, Chemistry Department, Technische Universität München
2011, Friedrich-Weygand Award 2011 of the Max-Bergmann Kreis
2011, Human Frontier Science Program (HFSP) long-term fellowship
2010, EMBO long-term fellowship
2010, Participation and invited talk at the Nobel-Laureates’ Meeting, Lindau, Germany
Research Interests
Prof. Hagn's research is focused on nuclear magnetic resonance (NMR) spectroscopy of membrane proteins. This protein class is essential for signal transduction and the transfer of proteins and small molecules across the biological membrane barrier. The key to understanding these processes is to study these systems in a native environment provided by a phospholipid bilayer. Biologically relevant systems of interest are mitochondrial membrane proteins, G-protein coupled receptors (GPCRs) and their associated G-proteins, which are involved in metabolic diseases, neurological disorders and cancer.
Demmer, Oliver; Frank, Andreas O.; Hagn, Franz; Schottelius, Margret; Marinelli, Luciana; Cosconati, Sandro; Brack-Werner, Ruth; Kremb, Stephan; Wester, Hans-Jürgen; Kessler, Horst: A Conformationally Frozen Peptoid Boosts CXCR4 Affinity and Anti-HIV Activity. Angewandte Chemie International Edition 51 (32), 2012, 8110-8113
Hagn, Franz: A structural view on spider silk proteins and their role in fiber assembly. Journal of Peptide Science 18, 2012, 357-365
Hagn, Franz; Lagleder, Stephan; Retzlaff, Marco; Rohrberg, Julia; Demmer, Oliver; Richter, Klaus; Buchner, Johannes; Kessler, Horst: Structural analysis of the interaction between Hsp90 and the tumor suppressor protein p53. Nat Struct Mol Biol 18 (10), 2011, 1086-1093
Hagn, Franz; Thamm, Christopher; Scheibel, Thomas; Kessler, Horst: pH-Dependent Dimerization and Salt-Dependent Stabilization of the N-terminal Domain of Spider Dragline Silk-Implications for Fiber Formation. Angewandte Chemie International Edition 50, 2010, 310-313
Hagn, Franz; Eisoldt, Lukas; Hardy, John G.; Vendrely, Charlotte; Coles, Murray; Scheibel, Thomas; Kessler, Horst: A conserved spider silk domain acts as a molecular switch that controls fibre assembly. Nature 465 (7295), 2010, 239-242
Retzlaff, Marco; Hagn, Franz; Mitschke, Lars; Hessling, Martin; Gugel, Frederik; Kessler, Horst; Richter, Klaus; Buchner, Johannes: Asymmetric Activation of the Hsp90 Dimer by Its Cochaperone Aha1. Molecular Cell 37, 2010, 344-354
Hagn, F.; Klein, C.; Demmer, O.; Marchenko, N.; Vaseva, A.; Moll, U. M.; Kessler, H.: BclxL Changes Conformation upon Binding to Wild-type but Not Mutant p53 DNA Binding Domain. Journal of Biological Chemistry 285, 2009, 3439-3450
Hassan, Lara; Lin, Liangcai; Sorek, Hagit; Sperl, Laura E.; Goudoulas, Thomas; Hagn, Franz; Germann, Natalie; Tian, Chaoguang; Benz, J. Philipp: Crosstalk of Cellulose and Mannan Perception Pathways Leads to Inhibition of Cellulase Production in Several Filamentous Fungi. mBio 10 (4), 2019 mehr…BibTeX
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Klöpfer, Kai; Hagn, Franz: Beyond detergent micelles: The advantages and applications of non-micellar and lipid-based membrane mimetics for solution-state NMR. Progress in Nuclear Magnetic Resonance Spectroscopy 114-115, 2019, 271-283 mehr…BibTeX
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Reif, Maria M.; Fischer, Moritz; Fredriksson, Kai; Hagn, Franz; Zacharias, Martin: The N-Terminal Segment of the Voltage-Dependent Anion Channel: A Possible Membrane-Bound Intermediate in Pore Unbinding. Journal of Molecular Biology 431 (2), 2019, 223-243 mehr…BibTeX
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Zeng, Baisen; Mou, Tung-Chung; Doukov, Tzanko I.; Steiner, Andrea; Yu, Wenxi; Papasergi-Scott, Makaia; Tall, Gregory G.; Hagn, Franz; Sprang, Stephen R.: Structure, Function, and Dynamics of the Gα Binding Domain of Ric-8A. Structure 27 (7), 2019, 1137-1147.e5 mehr…BibTeX
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2018
Goricanec, David; Hagn, Franz: NMR backbone and methyl resonance assignments of an inhibitory G-alpha subunit in complex with GDP. Biomolecular NMR Assignments 13 (1), 2018, 131-137 mehr…BibTeX
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Goricanec, David; Hagn, Franz: NMR backbone and methyl resonance assignments of an inhibitory G-alpha subunit in complex with GDP. Biomolecular NMR Assignments 13 (1), 2018, 131-137 mehr…BibTeX
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Miehling, Jonas; Goricanec, David; Hagn, Franz: A Split-Intein-Based Method for the Efficient Production of Circularized Nanodiscs for Structural Studies of Membrane Proteins. ChemBioChem 19 (18), 2018, 1927-1933 mehr…BibTeX
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Raltchev, Kolio; Pipercevic, Joka; Hagn, Franz: Production and Structural Analysis of Membrane-Anchored Proteins in Phospholipid Nanodiscs. Chemistry - A European Journal 24 (21), 2018, 5493-5499 mehr…BibTeX
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2017
Hagn, Franz; Nasr, Mahmoud L; Wagner, Gerhard: Assembly of phospholipid nanodiscs of controlled size for structural studies of membrane proteins by NMR. Nature Protocols 13 (1), 2017, 79-98 mehr…BibTeX
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Enthart, Andreas; Klein, Christian; Dehner, Alexander; Coles, Murray; Gemmecker, Gerd; Kessler, Horst; Hagn, Franz: Solution structure and binding specificity of the p63 DNA binding domain. Scientific Reports 6 (1), 2016 mehr…BibTeX
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Goricanec, David; Stehle, Ralf; Egloff, Pascal; Grigoriu, Simina; Plückthun, Andreas; Wagner, Gerhard; Hagn, Franz: Conformational dynamics of a G-protein α subunit is tightly regulated by nucleotide binding. Proceedings of the National Academy of Sciences 113 (26), 2016, E3629-E3638 mehr…BibTeX
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Nasr, Mahmoud L; Baptista, Diego; Strauss, Mike; Sun, Zhen-Yu J; Grigoriu, Simina; Huser, Sonja; Plückthun, Andreas; Hagn, Franz; Walz, Thomas; Hogle, James M; Wagner, Gerhard: Covalently circularized nanodiscs for studying membrane proteins and viral entry. Nature Methods 14 (1), 2016, 49-52 mehr…BibTeX
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2015
Milbradt, Alexander G.; Arthanari, Haribabu; Takeuchi, Koh; Boeszoermenyi, Andras; Hagn, Franz; Wagner, Gerhard: Increased resolution of aromatic cross peaks using alternate 13C labeling and TROSY. Journal of Biomolecular NMR 62 (3), 2015, 291-301 mehr…BibTeX
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2014
Hagn, Franz; Wagner, Gerhard: Structure refinement and membrane positioning of selectively labeled OmpX in phospholipid nanodiscs. Journal of Biomolecular NMR 61 (3-4), 2014, 249-260 mehr…BibTeX
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Linser, Rasmus; Gelev, Vladimir; Hagn, Franz; Arthanari, Haribabu; Hyberts, Sven G.; Wagner, Gerhard: Selective Methyl Labeling of Eukaryotic Membrane Proteins Using Cell-Free Expression. Journal of the American Chemical Society 136 (32), 2014, 11308-11310 mehr…BibTeX
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2013
Etzkorn, Manuel; Raschle, Thomas; Hagn, Franz; Gelev, Vladimir; Rice, Amanda J.; Walz, Thomas; Wagner, Gerhard: Cell-free Expressed Bacteriorhodopsin in Different Soluble Membrane Mimetics: Biophysical Properties and NMR Accessibility. Structure 21, 2013, 394-401 mehr…BibTeX
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