Scientific Report on TUM-IAS Fellowship
The TUM-IAS Focus Group Structural Membrane Biochemistry works on the development of tools for the high-resolution structure determination of membrane proteins in a native lipid bilayer environment by nuclear magnetic resonance (NMR) spectroscopy and other structural methods. Since membrane proteins are very difficult to study with structural methods, advanced strategies are required. full report
Short CV
- Since 10/2014
Rudolf Mößbauer Tenure Track Assistant Professor, Structural Membrane Biochemistry, Technische Universität München - 07/2010 -09/2014
Postdoctoral Associate, Harvard Medical School, Boston, USA.
Advisor: Prof. Dr. Gerhard Wagner, “Structural and functional studies of membrane proteins in phospholipid nanodiscs” - 11/2009-06/2010
Postdoctoral Associate, Institute of Organic Chemistry and Biochemistry, Technische Universität München, Germany.
Advisor: Prof. Dr. Horst Kessler, “NMR Spectroscopy of Molecular Chaperones, the p53 Tumorsuppressor Network and Spider Silk Proteins” - 01/2004-10/2009
Doctoral research, Institute of Organic Chemistry and Biochemistry, Technische Universität München, Germany.
Advisor: Prof. Dr. Horst Kessler „NMR Spectroscopy of Molecular Chaperones, the p53 Tumorsuppressor Network and Spider Silk Proteins“
Oct. 2009: Dr. rer. nat. (grade: summa cum laude) - 08/2003-11/2003
Intern, Aventis Pharma Germany GmbH, Frankfurt am Main at the division Drug Innovation and Approval, Chemistry.
“Binding studies of compounds to receptors and enzymes by NMR spectroscopy” - 10/2002-7/2003
Diploma, Institute of Biochemistry, Universität Bayreuth, Germany
Advisor: Prof. Dr. Jochen Balbach, (grade: summa cum laude) - 1998-2003
Study of Biochemistry, Universität Bayreuth, Germany
Selected Awards
- 2015, Helmholtz Young Investigators‘ Group
- 2013, TUM-IAS Hans Fischer Fellowship
- 2012, Arnold-Sommerfeld Award of the Bavarian Academy of Sciences
- 2011, Hans-Fischer Award 2011, Chemistry Department, Technische Universität München
- 2011, Friedrich-Weygand Award 2011 of the Max-Bergmann Kreis
- 2011, Human Frontier Science Program (HFSP) long-term fellowship
- 2010, EMBO long-term fellowship
- 2010, Participation and invited talk at the Nobel-Laureates’ Meeting, Lindau, Germany
Research Interests
Prof. Hagn's research is focused on nuclear magnetic resonance (NMR) spectroscopy of membrane proteins. This protein class is essential for signal transduction and the transfer of proteins and small molecules across the biological membrane barrier. The key to understanding these processes is to study these systems in a native environment provided by a phospholipid bilayer. Biologically relevant systems of interest are mitochondrial membrane proteins, G-protein coupled receptors (GPCRs) and their associated G-proteins, which are involved in metabolic diseases, neurological disorders and cancer.
Selected Publications
- Nasr, Mahmoud L; Baptista, Diego; Strauss, Mike; Sun, Zhen-Yu J; Grigoriu, Simina; Huser, Sonja; Plückthun, Andreas; Hagn, Franz; Walz, Thomas; Hogle, James M; Wagner, Gerhard: Covalently circularized nanodiscs for studying membrane proteins and viral entry. Nature Methods 14 (1), 2016, 49-52
- Etzkorn, Manuel; Raschle, Thomas; Hagn, Franz; Gelev, Vladimir; Rice, Amanda J.; Walz, Thomas; Wagner, Gerhard: Cell-free Expressed Bacteriorhodopsin in Different Soluble Membrane Mimetics: Biophysical Properties and NMR Accessibility. Structure 21, 2013, 394-401
- Hagn, Franz; Etzkorn, Manuel; Raschle, Thomas; Wagner, Gerhard: Optimized Phospholipid Bilayer Nanodiscs Facilitate High-Resolution Structure Determination of Membrane Proteins. J. Am. Chem. Soc. 135, 2013, 1919-1925
- Demmer, Oliver; Frank, Andreas O.; Hagn, Franz; Schottelius, Margret; Marinelli, Luciana; Cosconati, Sandro; Brack-Werner, Ruth; Kremb, Stephan; Wester, Hans-Jürgen; Kessler, Horst: A Conformationally Frozen Peptoid Boosts CXCR4 Affinity and Anti-HIV Activity. Angewandte Chemie International Edition 51 (32), 2012, 8110-8113
- Hagn, Franz: A structural view on spider silk proteins and their role in fiber assembly. Journal of Peptide Science 18, 2012, 357-365
- Hagn, Franz; Lagleder, Stephan; Retzlaff, Marco; Rohrberg, Julia; Demmer, Oliver; Richter, Klaus; Buchner, Johannes; Kessler, Horst: Structural analysis of the interaction between Hsp90 and the tumor suppressor protein p53. Nat Struct Mol Biol 18 (10), 2011, 1086-1093
- Hagn, Franz; Thamm, Christopher; Scheibel, Thomas; Kessler, Horst: pH-Dependent Dimerization and Salt-Dependent Stabilization of the N-terminal Domain of Spider Dragline Silk-Implications for Fiber Formation. Angewandte Chemie International Edition 50, 2010, 310-313
- Hagn, Franz; Eisoldt, Lukas; Hardy, John G.; Vendrely, Charlotte; Coles, Murray; Scheibel, Thomas; Kessler, Horst: A conserved spider silk domain acts as a molecular switch that controls fibre assembly. Nature 465 (7295), 2010, 239-242
- Retzlaff, Marco; Hagn, Franz; Mitschke, Lars; Hessling, Martin; Gugel, Frederik; Kessler, Horst; Richter, Klaus; Buchner, Johannes: Asymmetric Activation of the Hsp90 Dimer by Its Cochaperone Aha1. Molecular Cell 37, 2010, 344-354
- Hagn, F.; Klein, C.; Demmer, O.; Marchenko, N.; Vaseva, A.; Moll, U. M.; Kessler, H.: BclxL Changes Conformation upon Binding to Wild-type but Not Mutant p53 DNA Binding Domain. Journal of Biological Chemistry 285, 2009, 3439-3450
Publications as TUM-IAS-Fellow
