Structural Membrane Biochemistry

Prof. Franz Hagn has been a Rudolf Mößbauer Tenure Track Professor since October 2014. His research is focused on nuclear magnetic resonance (NMR) spectroscopy of membrane proteins. This protein class is essential for signal transduction and the transfer of proteins and small molecules across the biological membrane barrier. The key to understanding these processes is to study these systems in a native environment provided by a phospholipid bilayer. Biologically relevant systems of interest are mitochondrial membrane proteins, G-protein coupled receptors (GPCRs) and their associated G-proteins, which are involved in metabolic diseases, neurological disorders and cancer.

TUM-IAS funded doctoral candidate:
Joka Pipercevic, Structural Membrane Biochemistry
David Goricanec, Structural Membrane Biochemistry

TUM-IAS funded postdoctoral researcher:
Dr. Kai Fredriksson, Structural Membrane Biochemistry

Publications by the Focus Group

2019

Hassan, Lara; Lin, Liangcai; Sorek, Hagit; Sperl, Laura E.; Goudoulas, Thomas; Hagn, Franz; Germann, Natalie; Tian, Chaoguang; Benz, J. Philipp: Crosstalk of Cellulose and Mannan Perception Pathways Leads to Inhibition of Cellulase Production in Several Filamentous Fungi. mBio 10 (4), 2019 more…
Klöpfer, Kai; Hagn, Franz: Beyond detergent micelles: The advantages and applications of non-micellar and lipid-based membrane mimetics for solution-state NMR. Progress in Nuclear Magnetic Resonance Spectroscopy 114-115, 2019, 271-283 more…
Reif, Maria M.; Fischer, Moritz; Fredriksson, Kai; Hagn, Franz; Zacharias, Martin: The N-Terminal Segment of the Voltage-Dependent Anion Channel: A Possible Membrane-Bound Intermediate in Pore Unbinding. Journal of Molecular Biology 431 (2), 2019, 223-243 more…
Zeng, Baisen; Mou, Tung-Chung; Doukov, Tzanko I.; Steiner, Andrea; Yu, Wenxi; Papasergi-Scott, Makaia; Tall, Gregory G.; Hagn, Franz; Sprang, Stephen R.: Structure, Function, and Dynamics of the Gα Binding Domain of Ric-8A. Structure 27 (7), 2019, 1137-1147.e5 more…

2018

Goricanec, David; Hagn, Franz: NMR backbone and methyl resonance assignments of an inhibitory G-alpha subunit in complex with GDP. Biomolecular NMR Assignments 13 (1), 2018, 131-137 more…
Goricanec, David; Hagn, Franz: NMR backbone and methyl resonance assignments of an inhibitory G-alpha subunit in complex with GDP. Biomolecular NMR Assignments 13 (1), 2018, 131-137 more…
Miehling, Jonas; Goricanec, David; Hagn, Franz: A Split-Intein-Based Method for the Efficient Production of Circularized Nanodiscs for Structural Studies of Membrane Proteins. ChemBioChem 19 (18), 2018, 1927-1933 more…
Raltchev, Kolio; Pipercevic, Joka; Hagn, Franz: Production and Structural Analysis of Membrane-Anchored Proteins in Phospholipid Nanodiscs. Chemistry - A European Journal 24 (21), 2018, 5493-5499 more…

2017

Hagn, Franz; Nasr, Mahmoud L; Wagner, Gerhard: Assembly of phospholipid nanodiscs of controlled size for structural studies of membrane proteins by NMR. Nature Protocols 13 (1), 2017, 79-98 more…
Rodriguez Camargo, Diana C; Korshavn, Kyle J; Jussupow, Alexander; Raltchev, Kolio; Goricanec, David; Fleisch, Markus; Sarkar, Riddhiman; Xue, Kai; Aichler, Michaela; Mettenleiter, Gabriele; Walch, Axel Karl; Camilloni, Carlo; Hagn, Franz; Reif, Bernd; Ramamoorthy, Ayyalusamy: Stabilization and structural analysis of a membrane-associated hIAPP aggregation intermediate. eLife 6, 2017 more…

2016

Enthart, Andreas; Klein, Christian; Dehner, Alexander; Coles, Murray; Gemmecker, Gerd; Kessler, Horst; Hagn, Franz: Solution structure and binding specificity of the p63 DNA binding domain. Scientific Reports 6 (1), 2016 more…
Goricanec, David; Stehle, Ralf; Egloff, Pascal; Grigoriu, Simina; Plückthun, Andreas; Wagner, Gerhard; Hagn, Franz: Conformational dynamics of a G-protein α subunit is tightly regulated by nucleotide binding. Proceedings of the National Academy of Sciences 113 (26), 2016, E3629-E3638 more…
Nasr, Mahmoud L; Baptista, Diego; Strauss, Mike; Sun, Zhen-Yu J; Grigoriu, Simina; Huser, Sonja; Plückthun, Andreas; Hagn, Franz; Walz, Thomas; Hogle, James M; Wagner, Gerhard: Covalently circularized nanodiscs for studying membrane proteins and viral entry. Nature Methods 14 (1), 2016, 49-52 more…

2015

Milbradt, Alexander G.; Arthanari, Haribabu; Takeuchi, Koh; Boeszoermenyi, Andras; Hagn, Franz; Wagner, Gerhard: Increased resolution of aromatic cross peaks using alternate 13C labeling and TROSY. Journal of Biomolecular NMR 62 (3), 2015, 291-301 more…

2014

Hagn, Franz; Wagner, Gerhard: Structure refinement and membrane positioning of selectively labeled OmpX in phospholipid nanodiscs. Journal of Biomolecular NMR 61 (3-4), 2014, 249-260 more…
Linser, Rasmus; Gelev, Vladimir; Hagn, Franz; Arthanari, Haribabu; Hyberts, Sven G.; Wagner, Gerhard: Selective Methyl Labeling of Eukaryotic Membrane Proteins Using Cell-Free Expression. Journal of the American Chemical Society 136 (32), 2014, 11308-11310 more…

2013

Schilling, Franz: Novel Methods for NMR - Single-SHOT Correlation Spectroscopy and Diffusion of <sup>13</sup>C-Metabolites utilizing both Hyperpolarization and Optimal Control. Dissertation, 2013 more…

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