Ayyalusamy Ramamoorthy

Fellowship
Hans Fischer Senior Fellow

Institution
University of Michigan

Department
Chemistry and Biophysics

Host
Bernd Reif

Focus Group
Protein Misfolding and Amyloid Diseases

Short CV

  • 1996 - present
    Professor, Department of Chemistry & Biophysics, University of Michigan
  • 1993 - 1996
    Research Associate, Department of Chemistry, University of Pennsylvania
  • 1992 - 1993
    Scientist, Biometrology Laboratory, JEOL Ltd., Tokyo, Japan
  • 1989 - 1992
    Fellow Scientist, Central Leather Research Institute, India
  • 1990
    Ph.D. Chemistry, Indian Institute of Technology, Kanpur, India

Awards

2013 Robert W. Parry Collegiate Professor, University of Michigan

2012 Faculty Recognition Award, University of Michigan

2009 Fellow of the American Association for the Advancement of Science

2009 Willsmore Fellow, University of Melbourne

2009 Japan Society for the Promotion of Science Fellow

1999 – 2004 CAREER Development Award, National Science Foundation

Research Interests

Development of medicine to cure aging-related diseases (like Alzheimer's disease, type-2 diabetes, Parkinson disease etc) is severely limited by the challenges in fully understanding the misfolding, aggregation, and toxicity of amyloid proteins. Understanding high-resolution structures formed in the misfolding pathway and mechanisms of their toxicity can provide valuable insights into the factors that control amyloid diseases.

Our research group is primarily focused on solving the high-resolution structures of oligomeric intermediates of amyloid proteins (such as amyloid-beta and amylin (or IAPP)) and investigating their membrane interactions that are linked to cell toxicity by using a combination of solution and solid-state NMR and other biophysical techniques. Some of the specific topics include:
1. Misfolding, aggregation, and cell toxicity of human islet amyloid polypeptide (IAPP).
2. Mechanisms of membrane disruption by amyloid-beta (related to Alzheimer's Disease) and IAPP (implicated in type-2 diabetes).
3. Atomic-level resolution structures and dynamics of amyloid protein intermediates by NMR.
4. Effects of amyloid inhibitors on the structure and toxicity of abeta and IAPP.

Selected Publications

  • Lee, Dong-Kuk; Bhunia, Anirban; Kotler, Samuel A.; Ramamoorthy, Ayyalusamy: Detergent-type membrane fragmentation by MSI-78, MSI-367, MSI-594, and MSI-843 antimicrobial peptides and inhibition by cholesterol: a solid-state nuclear magnetic resonance study. Biochemistry 54 (10), 2015, 1897--1907 mehr… BibTeX Volltext ( DOI )
  • Brender, Jeffrey R.; Krishnamoorthy, Janarthanan; Sciacca, Michele F M; Vivekanandan, Subramanian; D'Urso, Luisa; Chen, Jennifer; {La Rosa}, Carmelo; Ramamoorthy, Ayyalusamy: Probing the sources of the apparent irreproducibility of amyloid formation: drastic changes in kinetics and a switch in mechanism due to micellelike oligomer formation at critical concentrations of IAPP. The journal of physical chemistry. B 119 (7), 2015, 2886--2896 mehr… BibTeX Volltext ( DOI )
  • Huang, Rui; Yamamoto, Kazutoshi; Zhang, Meng; Popovych, Nataliya; Hung, Ivan; Im, Sang-Choul; Gan, Zhehong; Waskell, Lucy; Ramamoorthy, Ayyalusamy: Probing the transmembrane structure and dynamics of microsomal NADPH-cytochrome P450 oxidoreductase by solid-state NMR. Biophysical journal 106 (10), 2014, 2126--2133 mehr… BibTeX Volltext ( DOI )
  • Kotler, Samuel A.; Walsh, Patrick; Brender, Jeffrey R.; Ramamoorthy, Ayyalusamy: Differences between amyloid-\textgreek{b} aggregation in solution and on the membrane: insights into elucidation of the mechanistic details of Alzheimer's disease. Chemical Society reviews 43 (19), 2014, 6692--6700 mehr… BibTeX Volltext ( DOI )
  • Hyung, Suk-Joon; DeToma, Alaina S.; Brender, Jeffrey R.; Lee, Sanghyun; Vivekanandan, Subramanian; Kochi, Akiko; Choi, Jung-Suk; Ramamoorthy, Ayyalusamy; Ruotolo, Brandon T.; Lim, Mi Hee: Insights into antiamyloidogenic properties of the green tea extract (-)-epigallocatechin-3-gallate toward metal-associated amyloid-\textgreek{b} species. Proceedings of the National Academy of Sciences of the United States of America 110 (10), 2013, 3743--3748 mehr… BibTeX Volltext ( DOI )
  • Yamamoto, Kazutoshi; Dürr, Ulrich H N; Xu, Jiadi; Im, Sang-Choul; Waskell, Lucy; Ramamoorthy, Ayyalusamy: Dynamic interaction between membrane-bound full-length cytochrome P450 and cytochrome b5 observed by solid-state NMR spectroscopy. Scientific reports 3, 2013, 2538 mehr… BibTeX Volltext ( DOI )
  • Ahuja, Shivani; Jahr, Nicole; Im, Sang-Choul; Vivekanandan, Subramanian; Popovych, Nataliya; Le Clair, Stéphanie V; Huang, Rui; Soong, Ronald; Xu, Jiadi; Yamamoto, Kazutoshi; Nanga, Ravi P.; Bridges, Angela; Waskell, Lucy; Ramamoorthy, Ayyalusamy: A model of the membrane-bound cytochrome b5-cytochrome P450 complex from NMR and mutagenesis data. The Journal of biological chemistry 288 (30), 2013, 22080--22095 mehr… BibTeX Volltext ( DOI )
  • Brender, Jeffrey R.; Salamekh, Samer; Ramamoorthy, Ayyalusamy: Membrane disruption and early events in the aggregation of the diabetes related peptide IAPP from a molecular perspective. Accounts of chemical research 45 (3), 2012, 454--462 mehr… BibTeX Volltext ( DOI )
  • Sciacca, Michele F M; Kotler, Samuel A.; Brender, Jeffrey R.; Chen, Jennifer; Lee, Dong-Kuk; Ramamoorthy, Ayyalusamy: Two-step mechanism of membrane disruption by A\textgreek{b} through membrane fragmentation and pore formation. Biophysical journal 103 (4), 2012, 702--710 mehr… BibTeX Volltext ( DOI )
  • Dürr, Ulrich H N; Gildenberg, Melissa; Ramamoorthy, Ayyalusamy: The magic of bicelles lights up membrane protein structure. Chemical reviews 112 (11), 2012, 6054--6074 mehr… BibTeX Volltext ( DOI )