Peer-Hendrik Kuhn

Peer-Hendrik (2013 Alumni)

Fellowship
Carl von Linde Junior Fellow

Institution
Technische Universität München

Department
Neuroproteomics

Host
Stefan Lichtenthaler

Focus Group
Proteomics

Short CV

Peer-Hendrik Kuhn studied medicine from 2001 until 2008 at Ludwig-Maximilians-Universität (LMU) in Munich. During his medical studies he was a scholarship holder of the Molecular Medicine program of LMU. He joined the laboratory of Christian Haass at the Adolf-Butenandt-Institute to study the role of proteases in Alzheimer’s disease. His state examination in medicine in 2008 was followed by the granting of a medical doctorate in 2009. After his medical studies, he joined the Medical Life Science and Technology program of the Technische-Universität München (TUM) and as well as the German Center of Neurodegenerative Diseases within the Hemholtz Association (DZNE) in Munich. In 2012 he received his doctorade. He has been a Carl von Linde Junior Fellow at TUM-IAS since the middle of 2013.

Selected Awards

2003-2005 Stipend of the Molecular Medicine MD program (FöFoLe of Ludwig-Maximilians University Munich)

Research Interests

In the past Peer-Hendrik Kuhn worked on the neurodegenerative disorder Alzheimer’s disease. During his research he especially focused his efforts on developing novel methods to answer important biological questions in the field. He developed a novel method called Secretome protein enrichment with click sugars (SPECS) which involves mass spectrometry, metabolic labeling of cells and bioorthogonal chemistry to analyze the secretome of primary cortical neurons. This in the end allowed the identification of substrates of the aspartylprotease BACE1 which is intimately linked to the pathogenesis of Alzheimer’s disease due to its initiative role in the generation of the neurotoxic peptide Aβ. Now that he has received the Carl von Linde Junior Fellowship he wants to develop this method further and apply it to other scientific fields like neoplasias of the hematopoietic system. This will allow a better understanding of cancer biology and additionally aid biomarker identification. Additionally these results might aid in therapeutic decisions in the near future. Furthermore he plans to develop additional methods which allow analyzing secreted and cell surface proteins directly from patient specimens.

Selected Publications

  • Hogl, Sebastian; van Bebber, Frauke; Dislich, Bastian; Kuhn, Peer-Hendrik; Haass, Christian; Schmid, Bettina; Lichtenthaler, Stefan F.: Label-free quantitative analysis of the membrane proteome of Bace1 protease knock-out zebrafish brains. Proteomics 13 (9), 2013, 1519-1527 mehr… BibTeX Volltext ( DOI )
  • Hogl, Sebastian; van Bebber, Frauke; Dislich, Bastian; Kuhn, Peer-Hendrik; Haass, Christian; Schmid, Bettina; Lichtenthaler, Stefan F.: Label-free quantitative analysis of the membrane proteome of Bace1 protease knock-out zebrafish brains. Proteomics 13 (9), 2013, 1519-1527 mehr… BibTeX Volltext ( DOI )
  • Müller-Rischart, Anne Kathrin; Pilsl, Anna; Beaudette, Patrick; Patra, Maria; Hadian, Kamyar; Funke, Maria; Peis, Regina; Deinlein, Alexandra; Schweimer, Carolin; Kuhn, Peer-Hendrik; Lichtenthaler, Stefan F.; Motori, Elisa; Hrelia, Silvana; Wurst, Wolfgang; Trümbach, Dietrich; Langer, Thomas; Krappmann, Daniel; Dittmar, Gunnar; Tatzelt, Jörg; Winklhofer, Konstanze F.: The E3 Ligase Parkin Maintains Mitochondrial Integrity by Increasing Linear Ubiquitination of NEMO. Molecular Cell 49 (5), 2013, 908-921 mehr… BibTeX Volltext ( DOI )
  • Colombo, Alessio; Wang, Huanhuan; Kuhn, Peer-Hendrik; Page, Richard; Kremmer, Elisabeth; Dempsey, Peter J.; Crawford, Howard C.; Lichtenthaler, Stefan F.: Constitutive α- and β-secretase cleavages of the amyloid precursor protein are partially coupled in neurons, but not in frequently used cell lines. Neurobiology of Disease 49, 2013, 137-147 mehr… BibTeX Volltext ( DOI )
  • Ivankov, D.N.; Bogatyreva, N.S.; Hönigschmid, P.; Dislich, B.; Hogl, S.; Kuhn, P.H.; Frishman, D.; Lichtenthaler, S.F.: QARIP, pp. a web server for quantitative proteomic analysis of regulated intramembrane proteolysis. Nucleic Acids Research 41, 2013 mehr… BibTeX
  • Kuhn, Peer-Hendrik; Koroniak, Katarzyna; Hogl, Sebastian; Colombo, Alessio; Zeitschel, Ulrike; Willem, Michael; Volbracht, Christiane; Schepers, Ute; Imhof, Axel; Hoffmeister, Albrecht; Haass, Christian; Roßner, Steffen; Bräse, Stefan; Lichtenthaler, Stefan F: Secretome protein enrichment identifies physiological BACE1 protease substrates in neurons. The EMBO Journal 31 (14), 2012, 3157-3168 mehr… BibTeX Volltext ( DOI )
  • Kuhn, Peer-Hendrik; Wang, Huanhuan; Dislich, Bastian; Colombo, Alessio; Zeitschel, Ulrike; Ellwart, Joachim W; Kremmer, Elisabeth; Roßner, Steffen; Lichtenthaler, Stefan F: ADAM10 is the physiologically relevant, constitutive α-secretase of the amyloid precursor protein in primary neurons. EMBO J 29 (17), 2010, 3020-3032 mehr… BibTeX Volltext ( DOI )
  • Kuhn, P.-H.; Marjaux, E.; Imhof, A.; De Strooper, B.; Haass, C.; Lichtenthaler, S. F.: Regulated Intramembrane Proteolysis of the Interleukin-1 Receptor II by  -, beta-, and  -Secretase. Journal of Biological Chemistry 282 (16), 2007, 11982-11995 mehr… BibTeX Volltext ( DOI )
  • Friedmann, Elena; Hauben, Ehud; Maylandt, Kerstin; Schleeger, Simone; Vreugde, Sarah; Lichtenthaler, Stefan F.; Kuhn, Peer-Hendrik; Stauffer, Daniela; Rovelli, Giorgio; Martoglio, Bruno: SPPL2a and SPPL2b promote intramembrane proteolysis of TNFα in activated dendritic cells to trigger IL-12 production. Nature Cell Biology 8 (8), 2006, 843-848 mehr… BibTeX Volltext ( DOI )